Tag Archive for: Bio4Energy Biopolymers and Biochemical Conversion

A so-called photobioreactor with microalgal content, in the laboratories of Christiane Funk and Martin Plöhn, both Bio4Energy. Photo by courtesy of Plöhn and Funk.

Researchers to Map Composition of Green Algae, Pave Way for Biotechnology Breakthroughs

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Bio4Energy’s research team dedicated to creating applications from green algae have won funds to investigate the composition of cell walls in microalgae. These are microscopic algae that are not visible to the unaided eye, used to produce algal biomass for use in biotechnology applications.

Christiane Funk and students at Umeå University, Sweden hope to develop much needed information on the mechanisms that govern buildup and breakdown of the cell wall in these Chlorophyta algae, with the aim of allowing researchers worldwide more easily to design biotechnology applications that are cost and energy efficient.

Last week, national funder Swedish Research Council – VR announced its decision to support a four-year project.

The researchers aim to find out which enzymes are involved in making the cell wall mouldable and the way in which this plasticity enables – or could enable – the green algae to cope with environmental stressors. They also aim to map the transport of carbon, which is known to fluctuate, inside the cell.

With this knowledge in hand, they want to look into ways to manipulate the cell wall for better outcomes in the design of biotechnology applications.

“This proposal aims to address a critical gap in research, by investigating the biosynthesis and modification of the cell wall of Chlorophyta microalgae. We will gain insights into the molecular mechanisms underlying the microalgal cell wall, its plasticity and perception of the environment”.

“Despite their promise as a sustainable feedstock for biotechnological applications, the use of microalgae has been hindered by high monetary and energy costs associated with the processing steps that follow cultivation, particularly the harvesting of algal biomass and extraction of valuable compounds”, Funk and colleagues wrote in their project application;

“This proposal aims to address this critical gap in research, by investigating the biosynthesis and modification of the cell wall of Chlorophyta microalgae. We will gain insights into the molecular mechanisms underlying the microalgal cell wall, its plasticity and perception of the environment”.

Over the last decade, Bio4Energy’s research teams studying microalgae have worked diligently to lay a foundation for production, scale up and demonstration of algal biomass for use in consumer products and as an agent in water purification in industrial facilities.

The two teams focus on green and blue-green microalgae, respectively. Francesco Gentili and colleagues, Swedish University of Agricultural Sciences, run development facilities in collaboration with regional energy utility Umeå Energi, at Dåva just off Umeå, in northern Sweden.

Project title: Not Just Another Brick in the Wall – Advancing Microalgal Biotechnology through Cell Wall Research

Project leader: Christiane Funk, Bio4Energy Biopolymers and Biochemical Conversion Technologies – Affiliation with Umeå University

Duration: 2025 – 2028

Related projects

Revitalising forest waste into microalgal and bacterial cellulose membranes with tailored properties for sustainable food packaging, Green Tech – Bio4Energy

Circular and sustainable production of bioplastics with the help of photosynthetic microorganisms – Proof of concept – Bio4Energy

Waste2Plastic – Production of bioplastic from algal biomass generated from wastewater – Bio4Energy

Related news

Microalgae that Thrive in Cold Climate Clean Wastewater, Give Biomass for Renewable Plastics – Bio4Energy

Sweden’s Bioeconomy Arena to Open by Early 2025: Bio4Energy Researchers Stopped by – Bio4Energy

Breakthrough Innovation: Hydrogels from Norwegian Kelp to Be Commercialised – Bio4Energy

Linn Berglund and Kristiina Oksman share a smile in the hydrogel development laboratory, with kelp on display. Photo by courtesy of Kristiina Oksman.

Innovator of Year Award to Bio4Energy Nanotechnology Experts

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Bio4Energy experts at nanotechnology have been selected to receive an ‘Innovator of the Year’ award by their home organisation Luleå University of Technology, Sweden for their continued efforts to develop bio-based solutions for industry. The award motivation highlights the creation of medical and health care applications as a particularly successful avenue.

LTU pro vice-chancellor Charlotte Winberg will be handing over the award at the university’s Innovation Day 5 November.

“We are very happy about the award and will focus even more on innovations so that our research can benefit society”, researches Kristina Oksman and Linn Berglund wrote in a press release from LTU.

The pair has successfully developed hydrogels from kelp seaweed that are being commercialised. Moreover, a smart dressing for wound healing, made by turning woody residue into nanofibre networks that take the form of a transparent gel—complemented by an equally transparent film overlay—is in preclinical testing.

The pair has successfully developed hydrogels from kelp seaweed that is being commercialised.

Moreover, a smart dressing for wound healing, made by turning woody residue into nanofibre networks that take the form of a transparent gel—complemented by an equally transparent film overlay—is in preclinical testing.

“What makes our innovations unique [are the fact] that they combine sustainability with versatility and functionality. We can tailor the biomaterials for different applications, making them useful in a variety of industries, from medicine to packaging”, associate professor Berglund said.

Last year, professor Oksman and Berglund made the 100 List hosted by the Royal Swedish Academy of Engineering Sciences for the invention of a sturdy composite material made from scrap textiles and plastic waste.

The List is published annually to indicate research innovations created at Swedish universities that could provide an economic and societal benefit, were they to be adopted by industry and commercialised.

Award motivation

“Oksman and Berglund’s work has great potential to contribute to societal benefits, particularly by reducing healthcare costs while also creating environmentally friendly alternatives for industry”, the press release said;

“Their bio-based solutions are not only energy-efficient to produce but can also replace oil-based materials, thereby reducing the use of fossil fuels and harmful chemicals”.

Recently, their Bionanocomposites’ research group has made bio-based films from woody residue after use as an underlying substance or layer for growing exotic mushrooms for human consumption. The mushrooms feed off this substrate layer to grow and break up the polymers of the wood during the while.

Recently, their Bionanocomposites’ research group has also made bio-based films from woody residue after use as an underlying substance or layer for growing exotic mushrooms for human consumption, in collaboration with Shaojun Xiong and colleagues at the Swedish University of Agricultural Sciences.

The mushrooms feed off this substrate layer to grow and break up the polymers of the wood during the while. This means that the researchers do not have to use chemicals to achieve their aim of breaking down the polymer lignin—the glue that binds together the main wood polymers cellulose, hemicellulose and lignin—since the mushroom carries out this service.

As part of the Bio4Energy research environment, Oksman and Berglund have gone from success to success. While Oksman was one of Bio4Energy’s founding research leaders, Berglund came in later as her student; rose through the ranks and never left since.

“Bio4Energy has been great for our research. We have had the freedom to invent new things. I do not think we could have done this without Bio4Energy”, Oksman told Bio4Energy Communications.

Contacts

Linn Berglund – Bio4Energy Biopolymers and Biochemical Conversion, affiliation with Luleå University of Technology

Kristiina Oksman – Bio4Energy Biopolymers and Biochemical Conversion, affiliation with Luleå University of Technology

Related projects

Sustainable packaging materials from renewable raw materials sources – Bio4Energy

Revitalising forest waste into microalgal and bacterial cellulose membranes with tailored properties for sustainable food packaging, Green Tech – Bio4Energy

Relation of wood structure and chemistry to nanocellulose extraction and properties – Bio4Energy

Development of energy-efficient processing technology of wood biomass into nanofibres and biocomposites through the use of fungal pre-treated substrates, accessing the sustainability goals – Bio4Energy

Investigating the electrochemical functionality of Norway spruce bark biochar and polymer composites – Bio4Energy

Utilising the natural composition of industrial bio-based residues for efficient separation of functional nanofibers – Bio4Energy

Related news

New Stride in Wound Healing Expected, as Researchers Add New Material for Medical Dressings – Bio4Energy

Breakthrough Innovation: Hydrogels from Norwegian Kelp to Be Commercialised – Bio4Energy

Inventions by Bio4Energy Researchers Highlighted by Royal Academy for Future Potential – Bio4Energy

In his PhD thesis, researcher Martin Plöhn lays out a scheme for wastewater treatment using microalgae. Photos by Anna Strom and Umea University photographers.

Microalgae that Thrive in Cold Climate Clean Wastewater, Give Biomass for Renewable Plastics

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A research report—covering five years of investigations—shows that microalgae grown in cold and dark conditions may not only be made to thrive on their own, but also remove the heavy metal content of industrial wastewater that conventional treatment plants do not filter out.

The high performing algal strain selected also turned out to produce ample carbohydrate biomass suitable for making bio-based plastics.

The academic research team behind the findings is based in northern Sweden; where winters are long, cold and dark. However, the cluster—including the research environment Bio4Energy and the MicroBioRefine project—have some of Scandinavia’s leading scientists in the field of developing biomass from blue-green algae as a renewable input material for making products.

The research report, by recent PhD graduate Martin Plöhn, will be released by Bio4Energy’s lead partner Umeå University as soon as details of its major findings have been cleared for publication in the chief biotechnology journal of a well-known publisher.

The researchers have identified a common and locally available strain, Chlorella vulgaris, as a top performer among microalgae when it comes to cleaning wastewater of cadmium, copper and lead. There was no additional source of energy or lighting added.

In a nutshell, the researchers have identified a common and locally available strain, Chlorella vulgaris, as a top performer among microalgae when it comes to cleaning wastewater of cadmium, copper and lead. The process has been tested in a research laboratory. There was no additional source of energy or lighting added to indoor room temperatures, daytime indoor (fluorescent) lighting and natural daylight.

Cleaning with microalgae after conventional wastewater treatment, to meet legal standards

Turned into a fully-fledged technology, the scheme would allow industries whose activities leave substantial amounts of wastewater in their wake, to shave the last one-to-two micrograms of heavy metals off wastewater already treated in a conventional treatment plant. The scheme comes with optional provisions for reuse in industry of the heavy metals thus recycled.

“Our microalgae can be used to treat wastewater to remove pollutants and produce freshwater…. We do not want to replace the conventional treatment system, but come in at the end and take away the heavy metal content that is still higher than the law”, doctor Plöhn told Bio4Energy Communications.

“Our microalgae can be used to remove pollutants and treat wastewater to produce freshwater… We do not want to replace the conventional treatment system, but come in at the end and take away the heavy metal content that is still higher than the law”.

In the second part of the microalgae project, Chlorella vulgaris again outperformed other strains tested when it came to producing polyhydroxybutyrate (PHB), a type of plastic, via bacterial breakdown of the biomass. The process has been tested in up to 25 litres of wastewater at a time, in a research laboratory.

Checking for unwanted emissions and scaling up

After successful proof of concept trials, the researchers have received expressions of interest for testing the concept on a larger scale from Bio4Energy partners at the RISE Research Institutes of Sweden. Plöhn and colleagues now are looking for industrial partners.

“We are looking for people who could be interested in the forest industry, with the message that we can add value… to existing processes”, he said.

The researchers collaborate with colleagues at the Swedish University of Agricultural Sciences to perform life-cycle assessment studies; to double check that their concept is sustainable in terms of minimising greenhouse gas emissions. Technically, the algae consume carbon dioxide down to net zero, but the researchers want to make sure that the system is water tight.

Dissertation in hand, Plöhn is not about to finish working on the project anytime soon. The microalgae also produce lipids and protein. Moreover there is the bio fertilizer route that remains to be explored.

“I see opportunities to explore this concept beyond carbohydrates. There will always be wastewater that needs to be treated. We need to use what we have right now”, he said.

Since late March Plöhn is a staff scientist at Umeå University and industry representatives are invited to contact him and the research team there for at least another nine months.

New for September 2024: News by NewsGram, Researchers aim to create biodegradable plastic – from algae (newsgram.com)

PhD Dissertation

Revealing the potential of Nordic microalgae — Turning waste streams into resources

Bio4Energy Contacts

Doctor Martin Plöhn — Affiliation with Umeå University

PhD Supervisor, Professor Christiane Funk — Affiliation with Umeå University

Related Projects

For more information

MicroBioRefine project

Bio4Energy Biopolymers and Biochemical Conversion

Carlos Martín Medina is new Bio4Energy coordinator for the Bio-based Industries' Consortium. Photo by ©AnnaStrom.©AnnaStrom

R&I on Bio Based in EU projects: ‘We Could Be More Proactive’

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Bio4Energy’s new coordinator for member organisation Bio-based Industries’ Consortium (BIC), Carlos Martín of Umeå University, is in Brussels, Belgium to network with industry members with a view to lay the foundations for an EU project.

February 8 BIC members met to network with companies, consultants and academics. The aim is jointly to apply for funds from the Circular Bio-based Joint Undertaking (CBE JU), which is a partnership between BIC and the European Union.

“On the Bio4Energy platforms we have expertise and knowledge of value for forming strong EU projects”, Martín said.

“We are interested in the topic Biotech routes to obtain bio-based chemicals or materials to replace animal-derived ones”.

“We are interested in the topic Biotech routes to obtain bio-based chemicals or materials to replace animal-derived ones”, he added.

As Martín points out, there is a lot at stake. The CBE JU partnership itself is worth €2 billion, according to its website.

It corresponds to the part of the Horizon Europe research and innovation (R&I) programme that is concerned with “advancing competitive circular bio-based industries”.

More specifically, it aims to accelerate the development of bio-based innovative solutions and their market deployment, while ensuring a high level of environmental performance of bio-based industrial systems.

“We could be more proactive toward partnerships and programs under Horizon 2020, including the [Joint Undertaking]”, Martin said;

“We have strong research that competes well with that of groups leading successful project proposals”.

Carlos Martín Medina is a long-standing member of the research environment Bio4Energy and its research platform Biopolymers and Biochemical Conversion. He has been part of developing state-of-the-art pre-treatment methods that allows for easier breakdown of woody biomass for conversion to liquid biofuels, together with current programme manager Leif Jönsson of Umeå University.

Having come to lean toward bio-based materials, Martín spearheaded a large collaboration project with Bolivia to make use of the abundant residue from the country’s production of quinoa, a staple food. In 2019, he took up a professorship at the Inland University of Applied Sciences in Norway, but continues to do research for Bio4Energy and Umeå University on investigating spent mushroom substrate as an input material for making products.

Contact

Carlos Martín

For more information

Circular Bio-based Europe Joint Undertaking

Bio4Energy Biopolymers and Biochemical Conversion

Related news

‘Getting Prepared to Have Right Material Base’: Chemistry in Biorefinery in New Report – Bio4Energy

Bio4Energy Researchers Meet to Usher in New Developments on Energy, Material Production – Bio4Energy

Quinoa Project Classifies New Building Block for Biorefinery – Bio4Energy

The timber we take out of forests should go toward constructing houses and furniture. Biorefinery is based on residues. Photo by Bent Christensen.Umea University

‘Getting Prepared to Have Right Material Base’: Chemistry in Biorefinery in New Report

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As economies are moving closer to a substantial fossil fuel phase-out, the need increases for a total overview of what the bio-based sector can bring to the table to replace it.

Bio4Energy researcher Carlos Martín Medina, Biopolymers and Biochemical Conversion, has spearheaded one such initiative giving an overview of how far we have come in terms of knowing the chemistry of the processes in factories where biofuels, “green” chemicals or bio-based materials are made: Biorefineries.

Together with colleagues from Spain and Italy, he has drawn together the latest advice from a range of international scientists on the Chemistry in Biorefineries and what substantial issues remain, in a new report.

“We are all concerned about [the consequences of using] fossil fuels. We need a clear idea of the post-petroleum era. We are getting prepared to have the right material base”, Martín told Bio4Energy Communications in an interview.

“What we are contributing with here is a representative overview of recent updates of known issues in biorefineries. These are novel contributions by first line scientists”, Martín said.

The Cuban native is one of Bio4Energy’s truly international PIs, bridging a position between Umeå University, Sweden and the Inland Norway University of Applied Sciences, Norway.

“What we are contributing with here is a representative overview of recent updates of known issues in biorefineries. These are novel contributions by first line scientists”.

As always when it comes to making commodities—even such that people will want to consume in the future—ventures have to be economically viable, as well as socially and environmentally sustainable.

“It is important to know the chemistry of [every] single process to be able to optimise and achieve higher yields and purity, and to avoid side reactions. In a biorefinery the first goal is to separate the three main components of biomass in the best way possible, so that each can be directed to different end products”, Martín explained.

Such products could be ethanol made from cellulose or resins made from lignin, he said. Although different input biomass materials are in focus in different parts of the world, the lesson contained in the themed collection of articles just out, in many cases are the same.

Is there enough biomass?

Martin’s answer to the question as to whether there is enough biomass for biorefinery production to make a substantial contribution in the post-petroleum era is a resounding “Yes.

“There are many different sources of residual plant biomass: Crop residues, forest residues, wood processing residues.

“Wood should mainly go into building materials and furniture manufacturing. We don’t want to clear out forests, [but instead] take advantage of materials that are not exploited today”.

The research environment Bio4Energy makes methods and tools for conducting biorefinery—a refinery based on biomass residues from various sectors to produce renewable fuel, materials and chemicals.  

For more information

Chemistry in Biorefineries is a themed collection of articles, published in an Advances journal by the Royal Society of Chemistry.

Editorial contacts

Carlos Martín Medina, Alejandro Rodríguez and Fabio Montagnaro

A model of the Vertisà AB vertical gardening module. Photo by courtesy of Vertisà AB.

Inventions by Bio4Energy Researchers Highlighted by Royal Academy for Future Potential

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Zeolite membranes for gas separation, vertical gardens and reuse of textiles to make composites. These are subjects of collaboration projects by Bio4Energy researchers who have made this year’s 100 List hosted by the Royal Swedish Academy of Engineering Sciences (IVA).

To make the List, it takes a research project deemed to have “great potential to be useful”. This usefulness is thought of as potential for commercialisation of the product or concept studied, for development of either business or methods, or for providing thought leadership.

Another key criterion is for the project leader or researchers on the project to have expressed interest in collaborating with industry or related entities to further develop their invention.

Membrane technology for gas separation in use, tends to be bulky, energy intensive and cost a lot. Bio4Energy researchers Jonas Hedlund and Liang Yu are perfecting and developing ultra-thin zeolite membranes that take up less space and use less energy to perform the separation. These membranes would provide a large cost reduction if rolled out on a large scale, according to the scientists.

With Vertisà Ltd, Rosario García-Gil and team propose a module vertical garden that can be added onto the exterior of a house and mimics a natural ecosystem. Complete with a built-in watering system, which has been patented, it is not only designed to help with greenhouse gas capture in cities, but also serves to insulate and beautify the wall it is attached to. The module is both low-technology and low cost, according to the project leader.

A new process has been invented, which allows for reuse of scrapped textiles as a component in a new, strong type of composite material based on a mixture of discarded textiles and plastics. Kristiina Oksman and co-workers used a piece of process equipment called extruder, to mix the cut fabrics with plastics. The resulting composite is two fifths textiles and costs less than the standalone plastic polymer.

Contacts

Jonas Hedlund and Liang Yu, Bio4Energy Catalysis and Separation, affiliation with Luleå University of Technology

Rosario García-Gil, Bio4Energy Forest-based Feedstocks, affiliation with the Swedish University of Agricultural Sciences

Kristiina Oksman, Bio4Energy Biopolymers and Biochemical Conversion, affiliation with Luleå University of Technology

Bio4Energy scientists Kristiina Oksman (left) and Linn Berglund are showing slivers of the brown kelp used as input material, at their research laboratory.LTU

Breakthrough Innovation: Hydrogels from Norwegian Kelp to Be Commercialised

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Bio4Energy researchers are behind a breakthrough innovation that can be used to make bio-based and biodegradable hydrogels.

Hydrogels are key components in materials used to restore or maintain human health such as wound healing, tissue engineering, artificial organs or everyday contact lenses.

The ingenuity of hydrogels lies in a dichotomy: While they are able to absorb and hold water, they do not decompose as a result.

However, as much as hydrogels are an indispensable part of modern medicine, today only synthetic hydrogels of the kind desired are available on the market and they are resource-intensive to produce, according to an article at the website of Luleå University of Technology, where the Bio4Energy researchers work.

Applying nanotechnology to brown algae grown in Norwegian waters, scientists Kristiina Oksman and Linn Berglund were able to skip steps that are paramount to making hydrogels of the synthetic kind. This means that the new bio-based technology requires less energy at production and generates less waste.

Nano-scale processing of the starting material also means that good quality hydrogel can be ascertained, as the cellulose is separated into ultra-small fibres and desirable qualities of the alginate salts are retained.

Alginor ASA, a Norwegian firm, has bought the resulting patent and are constructing a processing plant for this type of brown kelp, Laminaria Hyperborea.

“Alginor ASA wants to use the method to make full use of the harvest of Laminaria Hyperborea, or brown kelp, a species that is common in Norwegian waters”, professor Oksman said.

Contacts

Linn Berglund – Bio4Energy Biopolymers and Biochemical Conversion, affiliation with Luleå University of Technology

Kristiina Oksman – Bio4Energy Biopolymers and Biochemical Conversion, affiliation with Luleå University of Technology

Scientific article

No scientific literature has been disclosed.

Examples of Bio4Energy projects involving similar technologies can be found here: