Tag Archive for: algal biomass

A so-called photobioreactor with microalgal content, in the laboratories of Christiane Funk and Martin Plöhn, both Bio4Energy. Photo by courtesy of Plöhn and Funk.

Researchers to Map Composition of Green Algae, Paving Way for Biotechnology Breakthroughs

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Bio4Energy’s research team dedicated to creating applications from green algae have won funds to investigate the composition of cell walls in microalgae. These are microscopic algae that are not visible to the unaided eye, used to produce algal biomass for use in biotechnology applications.

Christiane Funk and students at Umeå University, Sweden hope to develop much needed information on the mechanisms that govern buildup and breakdown of the cell wall in these Chlorophyta algae, with the aim of allowing researchers worldwide more easily to design biotechnology applications that are cost and energy efficient.

Last week, national funder Swedish Research Council – VR announced its decision to support a four-year project.

The researchers aim to find out which enzymes are involved in making the cell wall mouldable and the way in which this plasticity enables – or could enable – the green algae to cope with environmental stressors. They also aim to map the transport of carbon, which is known to fluctuate, inside the cell.

With this knowledge in hand, they want to look into ways to manipulate the cell wall for better outcomes in the design of biotechnology applications.

“This proposal aims to address a critical gap in research, by investigating the biosynthesis and modification of the cell wall of Chlorophyta microalgae. We will gain insights into the molecular mechanisms underlying the microalgal cell wall, its plasticity and perception of the environment”.

“Despite their promise as a sustainable feedstock for biotechnological applications, the use of microalgae has been hindered by high monetary and energy costs associated with the processing steps that follow cultivation, particularly the harvesting of algal biomass and extraction of valuable compounds”, Funk and colleagues wrote in their project application;

“This proposal aims to address this critical gap in research, by investigating the biosynthesis and modification of the cell wall of Chlorophyta microalgae. We will gain insights into the molecular mechanisms underlying the microalgal cell wall, its plasticity and perception of the environment”.

Over the last decade, Bio4Energy’s research teams studying microalgae have worked diligently to lay a foundation for production, scale up and demonstration of algal biomass for use in consumer products and as an agent in water purification in industrial facilities.

The two teams focus on green and blue-green microalgae, respectively. Francesco Gentili and colleagues, Swedish University of Agricultural Sciences, run development facilities in collaboration with regional energy utility Umeå Energi, at Dåva just off Umeå, in northern Sweden.

Project title: Not Just Another Brick in the Wall – Advancing Microalgal Biotechnology through Cell Wall Research

Project leader: Christiane Funk, Bio4Energy Biopolymers and Biochemical Conversion Technologies – Affiliation with Umeå University

Duration: 2025 – 2028

Related projects

Revitalising forest waste into microalgal and bacterial cellulose membranes with tailored properties for sustainable food packaging, Green Tech – Bio4Energy

Circular and sustainable production of bioplastics with the help of photosynthetic microorganisms – Proof of concept – Bio4Energy

Waste2Plastic – Production of bioplastic from algal biomass generated from wastewater – Bio4Energy

Related news

Microalgae that Thrive in Cold Climate Clean Wastewater, Give Biomass for Renewable Plastics – Bio4Energy

Sweden’s Bioeconomy Arena to Open by Early 2025: Bio4Energy Researchers Stopped by – Bio4Energy

Breakthrough Innovation: Hydrogels from Norwegian Kelp to Be Commercialised – Bio4Energy

Martin Plöhn and Christiane Funk show green algae and sample plastic flakes in their research laboratory at Umeå University, Sweden. Photos by ©AnnaStrom.©AnnaStrom

Large Project Granted for Making Affordable Bio-based Plastics, Using Algae as Feed

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A consortium of Bio4Energy researchers has scored a grant for developing bio-based plastic to deliver prototypes of consumer products by project end, three years from now.

It involves a number of industrial and business partners who will provide either facilities and input material for experimental trials or develop consumer products, such as lampshade prototypes and a foam to go into packaging materials, respectively. The resulting products will be tested for their biodegradability.

It involves a number of industrial and business partners who will provide either facilities and input material for experimental trials or develop consumer products, such as lampshade prototypes and a foam to go into packaging materials, respectively. The resulting products will be tested for their biodegradability.

Global plastics production has exploded since the early 20th century and virtually all of it derives from fossil-based petrochemicals. In 2018, it stood at 359 million metric tons per annum.

At the end of life, over three fourths of plastics go into landfill. The breakdown of plastic made from petrochemicals generally takes hundreds of years and comes with leakage into the environment, especially for the kinds that degrade to microplastics during the composting process.

Plastic pollution has become an urgent global problem.

Innovation-to-consumer product value chain

In northern Sweden, Bio4Energy experts on the development and use of algae biomass for products and applications are proposing to tackle the issue head on by linking up actors in a research innovation-to-consumer product value chain.

The Swedish Energy Agency—which is not only a government agency, but also a research funder—has agreed to part sponsor the development of more affordable polyhydroxyalkanoate (PHA), which is a type of bio polyester that has the moldability of traditional plastics.

So far, PHA as a plastic alternative has had limited uptake, mainly because of the high cost of the feed for bacteria that make it. Here is where the Bio4Energy research comes in.

The scientists will identify strains of microalgae which, using sunlight and carbon dioxide, make biomass that the bacteria like to eat. The algae themselves will feed off industrial flue gases and wastewater produced at premises of regional energy utility Umeå Energi, which the green algae help clean during the while.

The scientists will identify strains of microalgae which, using sunlight and carbon dioxide (CO2), make biomass that the bacteria like to eat. The algae themselves will feed off industrial flue gases and wastewater produced at premises of regional energy utility Umeå Energi, which the green algae help clean during the while. The project also involves a utility that delivers drinking water, as well as handles sewage water treatment and waste recycling in the greater Umeå area; Vakin.

Algae research expert Christiane Funk will lead the project from Umeå University (UMU) and collaborate with Francesco Gentili, Swedish University of Agricultural Sciences (SLU), whose team operates development facilities at the Umeå Energi Dåva site. His colleague Carmen Cristescu will perform a life cycle assessment of the process. Bio4Energy programme manager Leif Jönsson’s group at UMU is also part of the project.

“We are going to use algae as feed for bacteria producing PHA, a type of bio polyester. The bacterial cultivation will be scaled up to litres by RISE Processum”, professor Funk said.

Membership company Processum at RISE Research Institutes of Sweden is one Bio4Energy’s strategic partners. Bio4Energy alumnus Pooja Dixit will lead this part of the work.

High cost of PHA limits market uptake

PHA as an alternative to petrochemical polymers for plastic production has had limited market uptake because of its high cost.

“It would be perfect to use PHA instead of plastic. We try to make it cheaper so that PHA can compete with fossil-based plastic and we also try to make the process more sustainable by using microalgae. We have to test which bacteria like which type of sugars [or carbohydrates] to produce PHA”, professor Funk said.

“It would be perfect to use PHA instead of plastic. We try to make it cheaper so that PHA can compete with fossil-based plastic and we also try to make the process more sustainable by using microalgae. We have to test which bacteria like which type of sugars to produce PHA”.

Downstream, two companies stand ready to turn the PHA into products.

In Stockholm, Interested Times Gang will take PHA from the project, to attempt 3D printing lampshades.

SME Cass Materials at Örnsköldsvik aim to mix the PHA with starch to improve an existing form of packing material in terms of its environmental footprint. The company describes the material as a “next generation bio-based foam that is lightweight with good mechanical strength and insulation properties for the packaging industry”.

Finally, Biocompost of Skellefteå is going to test the materials produced, particularly the ones that have a starch component, to see how long they take to biodegrade.

“We are going to work on the microalgae and the bacteria… and feed the carbohydrate to the bacteria in a two-step process”, Funk explained;

“We are going to test different algal strains [to ascertain] which produce the best feed for the bacteria”.

Globally, nine per cent of plastic waste is recycled and 12 per cent is incinerated. In countries that have ocean shorelines, each year between 4.8 million and 12.7 million metric tons of plastic waste are discarded into the sea. Source: Encylopaedia Britannica.

Project title: Waste2Plastic – Circular economy, recycling of CO2, nitrogen, phosphorus and water for bioplastics in a sustainable society

Funders: Swedish Energy Agency’s strategic innovation program RE:Source, which focuses on developing circular and resource-efficient material flows that are within planetary “boundaries”. The joint contribution of industrial partners is expected to match the SEA grant.

Bio4Energy research leaders involved

Christiane Funk, project manager and Leif Jönsson – Bio4Energy Biopolymers and Biochemical Conversion, affiliation with Umeå University

Francesco Gentili – Bio4Energy Environment and Nutrient Recycling, affiliation with the Swedish University of Agricultural Sciences

Carmen Cristescu – Bio4Energy Systems Analysis and Bioeconomy, affiliation with the Swedish University of Agricultural Sciences

Lalie Kossatz and Pooja Dixit – Processum at RISE

Business partners: Umeå Energi, Vakin, Cass Materials, ITG Studio, Biocompost

Related projects

Circular and sustainable production of bioplastics with the help of photosynthetic microorganisms – Proof of concept – Bio4Energy

Waste2Plastic – Production of bioplastic from algal biomass generated from wastewater – Bio4Energy

Related news

Microalgae that Thrive in Cold Climate Clean Wastewater, Give Biomass for Renewable Plastics – Bio4Energy

Sweden’s Bioeconomy Arena to Open by Early 2025: Bio4Energy Researchers Stopped by – Bio4Energy

Breakthrough Innovation: Hydrogels from Norwegian Kelp to Be Commercialised – Bio4Energy

In his PhD thesis, researcher Martin Plöhn lays out a scheme for wastewater treatment using microalgae. Photos by Anna Strom and Umea University photographers.

Microalgae that Thrive in Cold Climate Clean Wastewater, Give Biomass for Renewable Plastics

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A research report—covering five years of investigations—shows that microalgae grown in cold and dark conditions may not only be made to thrive on their own, but also remove the heavy metal content of industrial wastewater that conventional treatment plants do not filter out.

The high performing algal strain selected also turned out to produce ample carbohydrate biomass suitable for making bio-based plastics.

The academic research team behind the findings is based in northern Sweden; where winters are long, cold and dark. However, the cluster—including the research environment Bio4Energy and the MicroBioRefine project—have some of Scandinavia’s leading scientists in the field of developing biomass from blue-green algae as a renewable input material for making products.

The research report, by recent PhD graduate Martin Plöhn, will be released by Bio4Energy’s lead partner Umeå University as soon as details of its major findings have been cleared for publication in the chief biotechnology journal of a well-known publisher.

The researchers have identified a common and locally available strain, Chlorella vulgaris, as a top performer among microalgae when it comes to cleaning wastewater of cadmium, copper and lead. There was no additional source of energy or lighting added.

In a nutshell, the researchers have identified a common and locally available strain, Chlorella vulgaris, as a top performer among microalgae when it comes to cleaning wastewater of cadmium, copper and lead. The process has been tested in a research laboratory. There was no additional source of energy or lighting added to indoor room temperatures, daytime indoor (fluorescent) lighting and natural daylight.

Cleaning with microalgae after conventional wastewater treatment, to meet legal standards

Turned into a fully-fledged technology, the scheme would allow industries whose activities leave substantial amounts of wastewater in their wake, to shave the last one-to-two micrograms of heavy metals off wastewater already treated in a conventional treatment plant. The scheme comes with optional provisions for reuse in industry of the heavy metals thus recycled.

“Our microalgae can be used to treat wastewater to remove pollutants and produce freshwater…. We do not want to replace the conventional treatment system, but come in at the end and take away the heavy metal content that is still higher than the law”, doctor Plöhn told Bio4Energy Communications.

“Our microalgae can be used to remove pollutants and treat wastewater to produce freshwater… We do not want to replace the conventional treatment system, but come in at the end and take away the heavy metal content that is still higher than the law”.

In the second part of the microalgae project, Chlorella vulgaris again outperformed other strains tested when it came to producing polyhydroxybutyrate (PHB), a type of plastic, via bacterial breakdown of the biomass. The process has been tested in up to 25 litres of wastewater at a time, in a research laboratory.

Checking for unwanted emissions and scaling up

After successful proof of concept trials, the researchers have received expressions of interest for testing the concept on a larger scale from Bio4Energy partners at the RISE Research Institutes of Sweden. Plöhn and colleagues now are looking for industrial partners.

“We are looking for people who could be interested in the forest industry, with the message that we can add value… to existing processes”, he said.

The researchers collaborate with colleagues at the Swedish University of Agricultural Sciences to perform life-cycle assessment studies; to double check that their concept is sustainable in terms of minimising greenhouse gas emissions. Technically, the algae consume carbon dioxide down to net zero, but the researchers want to make sure that the system is water tight.

Dissertation in hand, Plöhn is not about to finish working on the project anytime soon. The microalgae also produce lipids and protein. Moreover there is the bio fertilizer route that remains to be explored.

“I see opportunities to explore this concept beyond carbohydrates. There will always be wastewater that needs to be treated. We need to use what we have right now”, he said.

Since late March Plöhn is a staff scientist at Umeå University and industry representatives are invited to contact him and the research team there for at least another nine months.

New for September 2024: News by NewsGram, Researchers aim to create biodegradable plastic – from algae (newsgram.com)

PhD Dissertation

Revealing the potential of Nordic microalgae — Turning waste streams into resources

Bio4Energy Contacts

Doctor Martin Plöhn — Affiliation with Umeå University

PhD Supervisor, Professor Christiane Funk — Affiliation with Umeå University

Related Projects

For more information

MicroBioRefine project

Bio4Energy Biopolymers and Biochemical Conversion